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yr digestion buffer  (Zymo Research)


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    Structured Review

    Zymo Research yr digestion buffer
    Yr Digestion Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/yr+digestion+buffer/YR+Digestion+Buffer/pm42000719-304-18-21
    Average 94 stars, based on 5 article reviews
    yr digestion buffer - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: Wastewater intelligence predicts the emergence of clinically-relevant and drug-resistant Candidozyma auris at healthcare facilities.
    Article Snippet: RNA sequencing and data analysis C. auris isolates were grown in YPD (Sigma Catalog #Y1375, 37°C, overnight) or SSDB (Thomas Scientific Catalog #CHM01P620, 42°C, 48 h). .. Cells were pelleted (2,900 × g, AR TI CL E IN P RE SS 5 min), resuspended in YR Digestion Buffer (Zymo Catalog #R1001-1) with 25 U Zymolyase (Zymo E1004), and incubated at 37°C for 60 min. Additional YR buffer and equal volume of ethanol (95-100%) were added and RNA was purified according to the manufacturer’s instructions for the RNeasy PowerMicrobiome Kit (Qiagen Catalog #26000-50), including DNase I treatment and elution in 54 μL RNase-free water. .. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation Kit (Illumina Catalog #20040534) with IDT RNA UD Indexes Set A (Illumina Catalog #20040553).

    Article Title: Wastewater intelligence predicts the emergence of clinically-relevant and drug-resistant Candida auris at healthcare facilities
    Article Snippet: C. auris isolates were grown in YPD (Sigma Catalog #Y1375, 37°C, overnight) or SSDB (Thomas Scientific Catalog #CHM01P620, 42°C, 48 h). .. Cells were pelleted (2,900 × g , 5 min), resuspended in YR Digestion Buffer (Zymo Catalog #R1001-1) with 25 U Zymolyase (Zymo E1004), and incubated at 37°C for 60 min. Additional YR buffer and equal volume of ethanal (95-100%) were added and RNA was purified according to the manufacturer’s instructions for the RNeasy PowerMicrobiome Kit (Qiagen Catalog #26000-50), including DNase I treatment and elution in 54 μL RNase-free water. .. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation Kit (Illumina Catalog #20040534) with IDT RNA UD Indexes Set A (Illumina Catalog #20040553).

    Purification:

    Article Title: Wastewater intelligence predicts the emergence of clinically-relevant and drug-resistant Candidozyma auris at healthcare facilities.
    Article Snippet: RNA sequencing and data analysis C. auris isolates were grown in YPD (Sigma Catalog #Y1375, 37°C, overnight) or SSDB (Thomas Scientific Catalog #CHM01P620, 42°C, 48 h). .. Cells were pelleted (2,900 × g, AR TI CL E IN P RE SS 5 min), resuspended in YR Digestion Buffer (Zymo Catalog #R1001-1) with 25 U Zymolyase (Zymo E1004), and incubated at 37°C for 60 min. Additional YR buffer and equal volume of ethanol (95-100%) were added and RNA was purified according to the manufacturer’s instructions for the RNeasy PowerMicrobiome Kit (Qiagen Catalog #26000-50), including DNase I treatment and elution in 54 μL RNase-free water. .. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation Kit (Illumina Catalog #20040534) with IDT RNA UD Indexes Set A (Illumina Catalog #20040553).

    Article Title: Wastewater intelligence predicts the emergence of clinically-relevant and drug-resistant Candida auris at healthcare facilities
    Article Snippet: C. auris isolates were grown in YPD (Sigma Catalog #Y1375, 37°C, overnight) or SSDB (Thomas Scientific Catalog #CHM01P620, 42°C, 48 h). .. Cells were pelleted (2,900 × g , 5 min), resuspended in YR Digestion Buffer (Zymo Catalog #R1001-1) with 25 U Zymolyase (Zymo E1004), and incubated at 37°C for 60 min. Additional YR buffer and equal volume of ethanal (95-100%) were added and RNA was purified according to the manufacturer’s instructions for the RNeasy PowerMicrobiome Kit (Qiagen Catalog #26000-50), including DNase I treatment and elution in 54 μL RNase-free water. .. Libraries were prepared using the Illumina Stranded mRNA Prep, Ligation Kit (Illumina Catalog #20040534) with IDT RNA UD Indexes Set A (Illumina Catalog #20040553).

    Centrifugation:

    Article Title: Emergent 3D genome reorganization from the stepwise assembly of transcriptional condensates
    Article Snippet: .. Cells were harvested by centrifugation, then lysed in YR Digestion buffer with Zymolyase using the YeaStar RNA kit (Zymo Research). .. Total RNA was isolated as per the YeaStar RNA kit manual and purified using the RNA Clean and Concentrator kit with in-column DNase I treatment (Zymo Research).



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